Viscotek P-columns for protein SEC
Viscotek P-Columns are designed for the separation of biopolymers such as proteins, enzymes and polysaccharides by Size Exclusion Chromatography (SEC). The packing material is a spherical porous silica gel coated with hydrophilic hydroxyl polymer. There are three types of columns for different molecular weight ranges.
Column size:
- Analytical columns
- 300mm L×7.8mm ID
- Guard column
- 50mm L×6.0mm ID
- Packing material
- Porous silica gel coated with
chemically-bonded hydroxyl polymer - Connecting screws
- Internally-threaded type, No.10 32 UNF
- Column material
- Stainless steel type SUS-316
- Shipping solvent
- H2O
- Max flow rate
- (analysis) 1.5mL/min (solvent exchange) 0.5mL/min
- Temperature Range
- 10 - 45 ℃
- pH range
- 3.0 - 7.5
- Organic solvents
- 0 – 100% Methanol or Acetonitrile
Table 1: Specifications
PN: CLM3025
- Grade
- P2500, Protein SEC Col
- Particle Size (μm)
- 5
- Theoretical Plate Number (per column)
- >21,000
- Exclusion Limit MW Protein
- 150,000
- Exclusion Limit MW Pullulan
- 60,000
- Max. pore size (A)
- 400
- Max pressure (MPa)
- 5.0
PN: CLM3026
- Grade
- P3000, Protein SEC Col
- Particle Size (μm)
- 5
- Theoretical Plate Number (per column)
- > 21,000
- Exclusion Limit MW Protein
- 700,000
- Exclusion Limit MW Pullulan
- 170,000
- Max. pore size (A)
- 1,000
- Max pressure (MPa)
- 5.0
PN: CLM3027
- Grade
- P4000, Protein SEC Col
- Particle Size (μm)
- 7
- Theoretical Plate Number (per column)
- > 16,000
- Exclusion Limit MW Protein
- (1,000,000)*
- Exclusion Limit MW Pullulan
- 500,000
- Max. pore size (A)
- 1,500
- Max pressure (MPa)
- 5.0
PN: CLM3028
- Grade
- PGuard
- Particle Size (μm)
- 7
- Theoretical Plate Number (per column)
- guard column
* Estimated values
More information
Featured presentations and application notes:
Application communication: Protein and polymer molecular size by GPC/SEC The main goal of most Gel Permeation/Size Exclusion Chromatography (GPC/SEC) experiments is to determine the molecular weight distribution (MWD) of the sample or compare molecular weights (MW) of several samples.
On-demand presentation: Protein characterization by triple detection Triple detection is used with size-exclusion chromatography to separate and characterize molecules such as proteins. Using triple detection allows the molecular weight and molecular size of a protein to be calculated independently of each other and retention time. This webinar will describe the technique and provide some example application data.
More application notes and online presentations are available to search for FREE in the Malvern Application Library and Presentations Database (Requires Free registration).
